Effect of Extraction effectivity of Senna alata L. Leaves on the Total Phenolic, Antioxidative, Antimicrobial and Antigoat Activity

Phytochemicals from natural product such as part of plants have been widely used as a potential source of herbal medication due to their bioactivity. Ketepeng cina (Senna alata L) is a small shrub that lives in tropical and subtropical regions and commonly used as medicinal plant to cure various diseases from infectious microbes. The current research was aimed to evaluate the extraction effectivity of Senna alata L leaves and the influence of the parameter to the total phenolic content, antioxidative, antimicrobial and antigout activity. Extraction of Senna alata L leaves was conducted by several methods, i.e maceration, ultrasonication and hot water extraction. The study revealed that each method give different yield of extraction and the most effective among those method is maceration, which produce highest yields of extracts (19.4%). Ultrasonic-assisted extraction (UAE) method also resulted in high yield but lower than that of maceration. The maceration method increases the yield by about 170% compared to hot water method. The extracts obtained from maceration method contain highest content of total phenolic (278.39mg GAE/g dry mass), thus demonstrate highest antioxidant activity (IC50 25.68mg/L), but lowest anti uric activity (reducing uric acid level by 85.25%). Maceration method was able to increase total phenolic content by about 128% and improve antioxidant activity by about 41%, compared to hot water method. However, UAE was excellent in producing phytochemicals which were responsible in anti uric activity, thus have the highest percentage in reducing UA level (90.72%), or increases by about 6.4%. compared to hot water method.