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You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research Panx1 blocker). Data were compared pairwise using the Mann-Whitney test. RESULTS: Exposure to 96hrs-CIS induced 4.0-fold increase in UF of WT mice (WT-CIS vs WT-Ctrl: 4.11±0.68-fold; n=15; p<0.001). This effect of stress on UF was blunted in Panx1-deficient mice (Panx1KO-CIS vs Panx1KO-Ctrl: 1.90±0.31-fold; n=9; p=0.04). Mechanical stimulation of mUC2-hTERT by 25% and 50% hypotonic shock or high K+ induced ATP release in amounts that were 3- to 4.5-fold higher than non-stimulated controls (p<0.05). These responses were blunted by the Panx1 channel blocker BB-FCF. Consistent with these findings, Yo-Pro dye uptake by mUC2-hTERT cells was higher when cells were mechanically stimulated in the presence of high K+ (compared to hypo 25%, p<0.0012; hypo 50%, p<0.0001, n=20 cells/field, 10 fields/dish). CONCLUSIONS: These findings support the involvement of Panx1 channels in mechanisms whereby urothelial barrier breach can lead to urinary IC/BPS symptoms by amplifying urothelial ATP release and signaling. These results provide basis for future preclinical studies to evaluate the potential use of Panx1 blockers to manage IC/BPS. Source of Funding: DoD W81XWH-21-1-0465 © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e961 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Anna Dukhovich More articles by this author Whitney Clearwater More articles by this author Marcia Urban-Maldonado More articles by this author Sylvia Suadicani More articles by this author Expand All Advertisement PDF downloadLoading ...
Dukhovich et al. (Mon,) studied this question.