Mp41-17 Circulating Tumour Cells and Cell-Free Dna Analysis for Monitoring Disease Progression in Localized Prostate Cancer

You have accessJournal of UrologyProstate Cancer: Markers I (MP41)1 May 2024MP41-17 CIRCULATING TUMOUR CELLS AND CELL-FREE DNA ANALYSIS FOR MONITORING DISEASE PROGRESSION IN LOCALIZED PROSTATE CANCER Marcel Figueras, Mercedes Ingelmo-Torres, Raquel Carrasco, Fiorella L. Roldán, Bernat Padullés, Claudia Mercader, Ascensión Gomez, Rita Pagés, Antonio Alcaraz, Lourdes Mengual, and Laura Izquierdo Marcel FiguerasMarcel Figueras , Mercedes Ingelmo-TorresMercedes Ingelmo-Torres , Raquel CarrascoRaquel Carrasco , Fiorella L. RoldánFiorella L. Roldán , Bernat PadullésBernat Padullés , Claudia MercaderClaudia Mercader , Ascensión GomezAscensión Gomez , Rita PagésRita Pagés , Antonio AlcarazAntonio Alcaraz , Lourdes MengualLourdes Mengual , and Laura IzquierdoLaura Izquierdo View All Author Informationhttps://doi.org/10.1097/01.JU.0001008896.93851.5b.17AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Liquid biopsy has been proposed to be useful for monitoring tumour progression in different solid tumours, including prostate cancer (PCa). Currently, prediction of disease progression after local treatment in localized PCa is based on clinicopathological parameters but none of them can accurately predict clinical outcome. This study focuses on evaluating the utility of circulating tumour cells (CTC) enumeration and cell-free DNA (cfDNA) concentration and fragmentation pattern to monitor biochemical recurrence (BCR) in localized PCa patients. METHODS: A total of 41 localized PCa patients in follow-up after local treatment were enrolled in the study. Blood samples were collected from each patient after surgery at the Urology Service from Hospital Clínic of Barcelona from May 2022 to May 2023. CTCs isolation was performed using IsoFlux system, stained using Isoflux Enumeration Kit and manually counted with a fluorescent microscope. Plasma cfDNA concentration and fragment size were quantified using Quant-it PicoGreen dsDNA Assay kit QIAamp and Agilent 2200 TapeStation System, respectively. Liquid biopsy data was compared with clinicopathological information. Kaplan-Meier curves were generated and a multivariable analysis using Cox regression was performed. RESULTS: Mean PSA value at diagnosis was 12.4ng/mL. Thirteen patients (31.7%) had pT2 stage and 26 (63,4%) pT3. In a mean follow-up of 32 months, 83% of patients developed BCR. A correlation was found between PSA values and CTCs count (p<0.001). Moreover, PCa patients with BCR had significantly higher number of CTCs (p=0.005). CTC count was able to discriminate between patients with and without BCR with a sensibility of 86% and a specificity of 71% (AUC=0.765; p=0.031). cfDNA fragmentation patterns correlated with PSA levels at diagnosis (p=0.047). In addition, a multivariant COX regression model including clinicopathological variables and liquid biopsy data showed that CTC enumeration (HR=16.765; 95% CI: 1.38-203.6; p=0.027) and pT (HR=4.113; 95% CI: 1.08-15.7; p=0.038) were independent factors of BCR during follow-up. CONCLUSIONS: CTC enumeration might be a useful predictor for BCR during localized PCa patients' follow-up. Validation of these results in larger series with longer follow-up is needed to elucidate the clinical significance of liquid biopsy in a localized PCa setting. The development of a non-invasive and sensitive tool able to identify relapse in PCa patients after local treatment could greatly improve PCa management and patients' clinical outcome. Source of Funding: None © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e681 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Marcel Figueras More articles by this author Mercedes Ingelmo-Torres More articles by this author Raquel Carrasco More articles by this author Fiorella L. Roldán More articles by this author Bernat Padullés More articles by this author Claudia Mercader More articles by this author Ascensión Gomez More articles by this author Rita Pagés More articles by this author Antonio Alcaraz More articles by this author Lourdes Mengual More articles by this author Laura Izquierdo More articles by this author Expand All Advertisement PDF downloadLoading ...