Mp42-12 the Impact of Microfluidic Sperm Selection on Assisted Reproductive Outcomes

You have accessJournal of UrologyInfertility: Therapy (MP42)1 May 2024MP42-12 THE IMPACT OF MICROFLUIDIC SPERM SELECTION ON ASSISTED REPRODUCTIVE OUTCOMES Ibrahim Samy, Adwoa Nyantakyi, Suks Minhas, Yau meen Thum, Tim Bracewell-Milnes, Jonathan Ramsay, James Nicopoullos, and Tet Yap Ibrahim SamyIbrahim Samy , Adwoa NyantakyiAdwoa Nyantakyi , Suks MinhasSuks Minhas , Yau meen ThumYau meen Thum , Tim Bracewell-MilnesTim Bracewell-Milnes , Jonathan RamsayJonathan Ramsay , James NicopoullosJames Nicopoullos , and Tet YapTet Yap View All Author Informationhttps://doi.org/10.1097/01.JU.0001008688.39367.31.12AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Sperm selection techniques have been developed to improve in-vitro fertilization (IVF) outcomes in male infertility. The quality of spermatozoa is considered very important, and having a high percentage of DNA-damaged spermatozoa in patients' semen might be a significant factor that decreases the success of assisted reproductive techniques (ART). Therefore a major challenge in the field of reproductive medicine is to guarantee the best possible quality of sperm used in ART. The most recent methods for preparing and selecting sperm involve microfluidic sperm selection using ZyMot. The aim of the study is to look at the outcomes of the micro-fluidic chamber sperm selection in a cohort of men with high DNA fragmentation. METHODS: A retrospective study of 88 couples with previous failed IVF cycles (n=37) or previous miscarriage and failure to conceive (n=51) and a male partner having high sperm DNA fragmentation who underwent IVF cycles using the ZyMot (DxNow, Maryland) between 2021-23 was performed Changes in DNA fragmentation pre and post Zymot, and IVF cycle outcomes including fertilisation, embryo transfer, pregnancy, miscarriage and live birth rates were monitored. DNA fragmentation was measured using the COMET Test (Examen, Belfast). All ZyMot cycles were through ICSI. RESULTS: DNA fragmentation results pre and post ZyMot were measured (n=34) by Comet with mean score of average comet score (ACS), low comet score (LCS), and high comet score (HCS), there was a 16% reduction in DNA fragmentation noted after ZyMot use. The mean age was measured of both male (40, range 31-58) and female (38, range 26-46) then cumulative pregnancy rate were illustrated below in comparison with cumulative live birth rate and ongoing pregnancy rate. CONCLUSIONS: Microfluidic techniques like ZyMot show potential for improving pregnancy rates in couples with high sperm DNA fragmentation who have had failed IVF cycles or recurrent miscarriage. This may be due to improved blastocyst conversion rate and reduced miscarriage. However, live birth/ongoing pregnancy rates remain static despite repeated cycles which may indicate a counter-effect with advancing egg age. Additional research is required within larger patient groups and in a prospective design to further substantiate these findings. Download PPT Source of Funding: No Funding © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e688 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Ibrahim Samy More articles by this author Adwoa Nyantakyi More articles by this author Suks Minhas More articles by this author Yau meen Thum More articles by this author Tim Bracewell-Milnes More articles by this author Jonathan Ramsay More articles by this author James Nicopoullos More articles by this author Tet Yap More articles by this author Expand All Advertisement PDF downloadLoading ...