PD51-01 PLASMA tsRNAs AS NOVEL DIAGNOSTIC BIOMARKERS FOR RENAL CELL CARCINOMA

You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology II (PD51)1 May 2024PD51-01 PLASMA tsRNAs AS NOVEL DIAGNOSTIC BIOMARKERS FOR RENAL CELL CARCINOMA Meng Ding, Wanqing Zhou, Wenli Diao, and Hongqian Guo Meng DingMeng Ding , Wanqing ZhouWanqing Zhou , Wenli DiaoWenli Diao , and Hongqian GuoHongqian Guo View All Author Informationhttps://doi.org/10.1097/01.JU.0001009360.84490.42.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Early diagnosis greatly benefits RCC patients with remarkable higher survival. Transfer RNA-derived small RNAs (tsRNAs) are stable and abundant in circulation, promising in non-invasive early diagnosis of cancer. Here, we explore the diagnosis values and biological functions of plasma tsRNAs in RCC for the first time. METHODS: Small RNA sequencing were performed to screen the tsRNAs expression profile in the plasma of RCC patients. A total of 129 healthy controls and 152 RCC patients were enrolled to identify differentially expressed plasma tsRNAs. ROC curve analysis and logistic regression analysis were used to evaluate the early diagnosis value of the plasma tsRNAs in RCC. RCC tissues analysis, cell function assays, and bioinformatics analysis were carried out to explore the biological function of tsRNAs in RCC. RESULTS: We identified the distinct expression profile of plasma tsRNAs in RCC patients, and further screened and validated five significantly decreased plasma tsRNAs in RCC patients. These five plasma tsRNAs had AUC values ranging from 0.7487 to 0.8549 (p<0.0001), and were all demonstrated to be independently risk factors for RCC (p<0.001). Among these, tRF-28-87R8WP9I1E0K obviously decreased in RCC tissues compared to paired normal kidney tissues, and over-expression of tRF-28-87R8WP9I1E0K significantly reduced the proliferation, self-new, migration, and invasion abilities of RCC cells. CONCLUSIONS: This study is the first systematic analysis of plasma tsRNA expression patterns in RCC patients, which provides novel potential biomarkers for accurate early diagnosis of RCC, and suggests a novel tsRNA-based therapeutic strategy for RCC. Download PPT Source of Funding: This work was supported by grants from the National Natural Science Foundation of China (81902571 to M.D., 82173160 to W.D., and 81972388 to H.G.), Nanjing Medical Science and technology development Foundation (ZKX22024 to M.D.) © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1064 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Meng Ding More articles by this author Wanqing Zhou More articles by this author Wenli Diao More articles by this author Hongqian Guo More articles by this author Expand All Advertisement PDF downloadLoading ...