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You have accessJournal of UrologyBladder Cancer: Basic Research p<0.001). No differences were reported in PDOs generation from fresh or frozen cells suspension (Fresh: 37% vs Freezing: 46%; p=0.51) and from peripheric or central tumor tissues (PT: 33% vs CT: 47%; p=0.38). Growth factors group 3 significantly increased PDOs generation (67%; p=0.02) (Figure 2-3). ATPlite assays reported a PDOs viability rate of 50% and 25%, with 5 µM and 10 µM CDDP, respectively, and a viability rate of 4% and 10% with 5 µM and 10 µM GEM. The combination of CDDP and GEM did not have a synergic effect, mainly due to the strong cytotoxic effect of GEM. CONCLUSIONS: We successfully developed successful culture conditions for BCa PDOs generation. By closely replicating the complexity of BCa tissue, PDOs seem to provide a promising tool to preclinically evaluate response to chemotherapy treatments, paving the way for the modern precision medicine. Download PPTDownload PPTDownload PPTDownload PPT Source of Funding: No © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1084 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Riccardo Mastroianni More articles by this author Carlotta Frascolla More articles by this author Sara Donzelli More articles by this author Claudio Pulito More articles by this author Andrea Russo More articles by this author Sabrina Strano More articles by this author Manuela Costantini More articles by this author Giovanni Blandino More articles by this author Giuseppe Simone More articles by this author Expand All Advertisement PDF downloadLoading ...
Mastroianni et al. (Mon,) studied this question.