Mp76-14 Prophylactic Dips and Irrigation Solutions Can Considerably Alter the Antimicrobial Efficacy of Inflatable Penile Prosthesis Hydrophilic Surface Coatings

You have accessJournal of UrologySexual Function/Dysfunction: Surgical Therapy II (MP76) 1 May 2024MP76-14 PROPHYLACTIC DIPS AND IRRIGATION SOLUTIONS CAN CONSIDERABLY ALTER THE ANTIMICROBIAL EFFICACY OF INFLATABLE PENILE PROSTHESIS HYDROPHILIC SURFACE COATINGS Brian H. Im, Sohan Shah, Rishabh Simhal, Samone Guillame, Rachel Evans, Carlos Perez, Noreen J. Hickok, and Paul H. Chung Brian H. ImBrian H. Im, Sohan ShahSohan Shah, Rishabh SimhalRishabh Simhal, Samone GuillameSamone Guillame, Rachel EvansRachel Evans, Carlos PerezCarlos Perez, Noreen J. HickokNoreen J. Hickok, and Paul H. ChungPaul H. Chung View All Author Informationhttps: //doi. org/10. 1097/01. JU. 0001009484. 98400. 42. 14AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: To date, the antimicrobial efficacy of 0. 05% chlorohexidine gluconate (CHG) as a prophylactic dip or an intraoperative irrigation solution has not been compared to that of an antibiotic. We aim to characterize the antimicrobial efficacy of 0. 05% CHG and vancomycin/gentamicin (VG) as both an intraoperative irrigation solution and an antiseptic dip on inflatable penile implant (IPP) hydrophilic surfaces. METHODS: Sterile 8mm diameter IPP pieces underwent the following treatment protocols. 1: IPP pieces were submerged in normal saline (NS), 0. 05% CHG, or a solution of 2 mg/mL vancomycin and 160 μg/mL gentamicin (VG) for 3 minutes. 2: IPP pieces were submerged in NS, 0. 05% CHG, or VG for 3 minutes, rinsed in NS for five seconds, then re-submerged in a separate well containing NS, 0. 05% CHG, or VG for 2 minutes. The pieces were rinsed in NS, then incubated in 10⁵ CFU/mL of methicillin-sensitive Staphylococcus aureus for 48 hours. Samples were rinsed in PBS, after which adherent bacteria was removed by shaking in 0. 3% Tween-20, serially diluted, plated onto 3M PetriFilms, and counted. Mann-Whitney U tests were performed to compare outcomes, p<0. 05 considered significant. RESULTS: Average bacterial counts with 95% CIs are plotted in Figures 1a and 1b. Although pre-dipping the implant in 0. 05% CHG and VG resulted in ∼2 log and ∼5 log decreases in bacterial counts respectively (0. 05% CHG: p<0. 01; VG: p<0. 01), the introduction of NS or CHG to an implant dipped in VG resulted in a near-complete loss of antimicrobial efficacy, with a 0. 5 log decrease (NS) and a slight increase (CHG) in bacterial growth relative to NS controls. However, VG dipping followed by VG irrigation resulted in retention of antimicrobial properties, with a comparable 5 log decrease in bacterial counts. CONCLUSIONS: VG confers greater antimicrobial efficacy as a prophylactic dip compared to 0. 05% CHG. Additionally, irrigation with NS or 0. 05% CHG significantly alters the efficacy of antiseptic solutions bound to IPP hydrophilic surfaces. Download PPT Source of Funding: PHC receives research support from and consults for Coloplast and Boston Scientific © 2024 by American Urological Association Education and Research, Inc. FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1248 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc. Metrics Author Information Brian H. Im More articles by this author Sohan Shah More articles by this author Rishabh Simhal More articles by this author Samone Guillame More articles by this author Rachel Evans More articles by this author Carlos Perez More articles by this author Noreen J. Hickok More articles by this author Paul H. Chung More articles by this author Expand All Advertisement PDF downloadLoading. . .