Pd16-02 Increased Spatial Coupling of Integrin and Collagen Iv in the Immunoresistant Clear Cell Renal Cell Carcinoma Tumor Microenvironment

You have accessJournal of UrologyKidney Cancer: Basic Research COL4A1 (gene for collagen IV) and ITGAV (gene for integrin αv-subunit) in the stroma (FDR=0.056). Expression of these genes were highest amongst fibroblasts, tumor cells, and endothelial cells. On mIF validation testing, integrin αv positive cells and cells that were dually positive for integrin αv and smooth muscle actin (a fibroblast marker) were more abundant in the IO exposed stroma compared to IO naïve stroma (p=0.001 and p<0.001). CONCLUSIONS: We found a shift in the abundance of immune cells in the ccRCC TIME following IO treatment. Additionally, we saw significant autocorrelation of two transcripts associated with the EMT pathway, ITGAV and COL4A1. Increased abundance of integrin αv positive cells was confirmed on mIF validation testing. Source of Funding: 2023 Moffitt Team Science-Miles for Moffitt Award, and the Cancer Center Support Grant at the H. Lee Moffitt Cancer Center and Research Institute, an NCI designated Comprehensive Cancer Center (P30 CA 076292) © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e365 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Mitchell T. Hayes More articles by this author Alex Soupir More articles by this author Taylor Peak More articles by this author Oscar Ospina More articles by this author Jonathan Nguyen More articles by this author Nicholas H. Chakiryan More articles by this author Natasha Francis More articles by this author Paola Ramos Echevarria More articles by this author Kenneth Y. Tsai More articles by this author Jodi Balasi More articles by this author Yamila Caraballo Peres More articles by this author Jasremon Dhillon More articles by this author Lindsey A. Martinez More articles by this author Warren E. Gloria More articles by this author James Mule More articles by this author Brooke L. Fridley More articles by this author Brandon J. Manley More articles by this author Expand All Advertisement PDF downloadLoading ...