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This project aims to create and verify a method using high-performance liquid chromatography-mass spectrometry (HPLC-MS) to measure the amount of ritonavir in human plasma. Saquinavir will be used as the internal standard for this analysis. Chromatographic isolation was processed HYPURITY ADVACE 50 × 4.6 mm, 5 μm (Make: Thermo scientific) analytical column with mobile phase composition of methanol and ammonium acetate 5 mm buffer in the ratio of 85:15% v/v. Detection was processed in a positive ionic approach and the parent and product ion transitions were monitored at 721.30/296.10 for ritonavir and 671.30/570.30 for saquinavir (API 2000). The measurement of the linearity curve for regression analysis The correlation coefficient (r) exhibited a remarkable value of over 0.99 within the concentration range of 8.004 to 1600.001 ng/mL for ritonavir. All eight batches showed no significant matrix effect. It was found that the standardized matrix factor had a precision of 1.90% at the LQC level and 2.38% at the HQC level. It was 0.992 for LQC and 1.005 for HQC when the IS factor was taken into account. Ritonavir had a general recovery rate of 89.07%, with a range of accuracy from 0.85 to 2.55%. The internal standard drug saquinavir had an average recovery rate of 90.18%, with a range of accuracy from 1.89 to 3.40%. The developed method was successfully validated and can be utilized for the assessment of ritonavir in biological matrices in industries, forensic labs, quality control labs, and bioavailability studies.
Katthala et al. (Mon,) studied this question.