Key points are not available for this paper at this time.
Abstract Eicosanoids are synthesized from phospholipids by the catalytic activity of phospholipase A 2 (PLA 2 ). Even though several PLA 2 s are encoded in the genome of different insect species, their physiological functions are not clearly discriminated. This study identified four PLA 2 genes encoded in the western flower thrips, Frankliniella occidentalis . Two PLA 2 s ( Fo-PLA 2 C and Fo-PLA 2 D ) are predicted to be secretory while the other two PLA 2 s ( Fo-PLA 2 A and Fo-PLA 2 B ) are intracellular. All four PLA 2 genes were expressed in all developmental stages, of which Fo-PLA 2 B and Fo-PLA 2 C were highly expressed in larvae while Fo-PLA 2 A and Fo-PLA 2 D were highly expressed in adults. Their expressions in different tissues were also detected by fluorescence in situ hybridization. All four PLA 2 s were detected in the larval and adult intestines and the ovary. Feeding double-stranded RNAs specific to the PLA 2 genes specifically suppressed the target transcript levels. Individual RNA interference (RNAi) treatments led to significant developmental retardation, especially in the treatments specific to Fo-PLA 2 B and Fo-PLA 2 D . The RNAi treatments also showed that Fo-PLA 2 B and Fo-PLA 2 C expressions were required for the induction of immune-associated genes, while Fo-PLA 2 A and Fo-PLA 2 D expressions were required for ovary development. These results suggest that four PLA 2 s are associated with different physiological processes by their unique catalytic activities and expression patterns.
Esmaeily et al. (Mon,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: