Serum Anti-Mullerian hormone levels in Turkish girls aged 18 and younger for ovarian reserve determination

Objective: Our aim was to show that AMH may be used as a quantitative marker of ovarian reserve in Turkish girls aged 18 and younger and establish the reference values for AMH in Turkish girls.Material and Methods: This retrospective study includes girls between ages of 8-18, without premature ovarian failure or without genetic factors resulting in ovarian dysgenesis.Blood specimens were collected after overnight fasting early in the morning during the early follicular phase.Measurement of serum levels of gonadotropins and AMH is done.Mean serum AMH levels of different age groups and best fitting curve representing AMH percentiles (10 th , 25 th , 50 th , 75 th , 90 th ) were calculated.Results: We identified 785 Turkish girls with mean age of 16.16 ± 1.90.The girls were divided into seven age groups.The mean serum AMH level for total cohort is 5.20 ± 4.19 ng/mL.There was statistically significant difference between the mean values of AMH in age groups as follows: £12 and 17-£18 (p=0.011).The best fitting curves for AMH percentiles were 4 th order polynomial functions.There is statistically significant correlation of AMH with age and FSH levels (r=0.148,p=0.000 and r=-0.092,p=0.010). Conclusion:Our results reflect the real-life data for serum AMH values in Turkish girls.Our nomogram may be useful for counseling the adolescents about their ovarian reserve and diagnosing other gynecological diseases.A longitudinal study is necessary for improving the predictive value of AMH values in girls aged 18 and younger.