Cytotoxicity of Glyphosate Studied in Selected Human Cell Lines

firstₚage Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Cytotoxicity of Glyphosate Studied in Selected Human Cell Lines † by Agata Jabłońska-TrypućAgata Jabłońska-Trypuć SciProfiles Scilit Preprints. org Google Scholar 1, *, Gabriela SokołowskaGabriela Sokołowska SciProfiles Scilit Preprints. org Google Scholar 1, Monika NaumowiczMonika Naumowicz SciProfiles Scilit Preprints. org Google Scholar 2, Urszula WydroUrszula Wydro SciProfiles Scilit Preprints. org Google Scholar 1 and Elżbieta WołejkoElżbieta Wołejko SciProfiles Scilit Preprints. org Google Scholar 1 1 Department of Chemistry, Biology and Biotechnology, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, Wiejska 45E Street, 15-351 Białystok, Poland 2 Department of Physical Chemistry, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K Street, 15-245 Bialystok, Poland * Author to whom correspondence should be addressed. † Presented at the 1st International Electronic Conference on Toxics, 20–22 March 2024; Available online: https: //sciforum. net/event/IECTO2024. Proceedings 2024, 102 (1), 13; https: //doi. org/10. 3390/proceedings2024102013 Published: 3 April 2024 Download keyboardₐrrowdown Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: glyphosate; cytotoxicity; cancer; toxicology Introduction: Glyphosate is a phosphonate herbicide that has been used for 35 years, and it is still the most widely used herbicide in the world. The development of modern methods of in vitro toxicological analysis and the obvious increase in exposure to glyphosate have made it an increasingly studied xenobiotic. The widespread use of pesticides, especially glyphosate, causes large amounts to be released into the environment, where they pose a threat to entire ecosystems. Methods: In our research, we focused on the best research model from the point of view of toxicology, in which human cell lines were used in order to test the cytotoxicity of glyphosate. The tested concentrations of this compound reflected the level of human exposure to this active substance and ranged from 0. 01 mM to 10 mM. The analyzes were conducted on the human cell lines: MCF-7, T98G and DLD-1. In order to estimate the cytotoxicity of this herbicide, an MTT assay was used. Results: A significant increase in cancer cell viability was observed under the influence of the studied concentrations of glyphosate. The effect was significant, especially under the influence of the lower concentrations studied. Conclusions: The obtained results indicate the carcinogenic and tumor cell-stimulating effects of glyphosate. This indicates the need for the prudent use and monitoring of glyphosate in the environment due to its potentially hazardous effects on human health. Author ContributionsConceptualization, A. J. -T. , G. S. and E. W. ; methodology, A. J. -T. , G. S. , U. W. , E. W. and M. N. ; software, A. J. -T. and U. W. ; validation, E. W. and U. W. ; formal analysis, A. J. -T. and M. N. ; investigation, A. J. -T. , G. S. , M. N. , U. W. and E. W. ; resources, A. J. -T. , E. W. and U. W. ; data curation, G. S. , E. W. and U. W. ; writing—original draft preparation, A. J. -T. , G. S. , E. W. and U. W. ; writing—review and editing, A. J. -T. , G. S. , E. W. , U. W. and M. N. ; visualization, E. W. , U. W. and A. J. -T. ; supervision, A. J. -T. ; project administration, A. J. -T. ; funding acquisition, A. J. -T. , E. W. and U. W. All authors have read and agreed to the published version of the manuscript. FundingThis work was financially supported by the Ministry of Education and Science, Poland, under the research project number WZ/WB-IIŚ/6/2022. Institutional Review Board StatementNot applicable. Informed Consent StatementNot applicable. Data Availability StatementThe data presented in this study are available on a reasonable request from the corresponding author. Conflicts of InterestThe authors declare no conflicts of interest. 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Cytotoxicity of Glyphosate Studied in Selected Human Cell Lines. Proceedings. 2024; 102 (1): 13. https: //doi. org/10. 3390/proceedings2024102013 Chicago/Turabian Style Jabłońska-Trypuć, Agata, Gabriela Sokołowska, Monika Naumowicz, Urszula Wydro, and Elżbieta Wołejko. 2024. "Cytotoxicity of Glyphosate Studied in Selected Human Cell Lines" Proceedings 102, no. 1: 13. https: //doi. org/10. 3390/proceedings2024102013 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.