Understanding of potentization process of homoeopathic medicine of Aurum Metallicum and its characterisation

Objectives: The effect of homoeopathic potentization increases with dilution. The physical and chemical properties of the homoeopathy solution change as a function of the concentration of nanoparticles (NPs). The succussion process is also vital in homoeopathy. However, none of these factors, along with the container of the medicine, have been well-researched to date. Material and Methods: We studied the systematic ultra-low dilution (up to 10 200 times) effect of homoeopathic remedy of Aurum Metallicum (Gold) NP colloidal solution to determine the mechanism of colloidal formation in such extreme dilution. Several material characterizations were also performed to consolidate our results. Results: Optical spectroscopy confirmed the presence of NPs with narrow size distribution and high surface activity in higher dilution (up to 10 200 times) homoeopathic solution. The particle size analysis of the serially diluted solution showed that the size distribution becomes narrow with increasing dilution. Transmission electron microscopy confirmed the presence of NPs in solutions up to 10 200 times dilution. Conclusion: The process of dilution introduces an active hydrocarbon layer on these NPs. The experimental study further confirmed theoretical calculations. The understanding of such extreme dilutions can be utilized in biomedical applications, especially in homoeopathic medicine.