Pseudomonas aeruginosa: Isolation and Characterization

A total samples were collected from patients who attended the main hospitals in Duhok city during the period from 8th May 2022 to 28th February 2023. Bacterial identification, and antimicrobial susceptibility were tested using the traditional methods and confirmed by VITEK 2 compact system. Fifty selected P. aeruginosa isolates were molecularly detected by conventional PCR assay using specific primers and sequencing of aac(6')-lb gene. For the prevalence of P. aeruginosa, 160 isolates were isolated from 670 samples including, burn 48(7.17%), ear 39(5.82%), wound 25(3.73%), urine 27(4.03%), and sputum 21(3.13%). Regarding antibiotic-resistant pattern, resistance was noticed to tobramycin, gentamicin, and amikacin showed resistance of 64.4%, 57.5%, and 51.9%, respectively. On molecular study, 50 isolates from 160 were selected for PCR assay. Selected isolates were confirmed by PCR assay that 46/50 (92%) were positive after aac(6')-lb gene with size 472 bp amplification, and have got clear bands on agarose gel 1% and electrophoresis, 10 PCR positive samples were selected to know the sequencing of aac(6')-lb genes. Moreover, all sequences were submitted to National Center for Biotechnology Information blast, recorded into Genbank, and got accession numbers for for aac(6')-lb (OQ538204, OQ538205, OQ538206, OQ538207, OQ538208, OQ538209, OQ538210, OQ538211, OQ538212, and OQ538213).The aac(6')-lb samples were identical (99-100%) to the references sequence.