Key points are not available for this paper at this time.
This protocol is used for eDNA metabarcoding of the 18S SSU rRNA Gene (Balzano et al 2015) using Pair-End Illumina Miseq. Sequencing. As part of the Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), to genetically characterize plankton communities in the Northern Salish Sea since 2015. This protocol is developed to provide taxonomic annotations of Eukaryote Nuclear DNA.
Carvalho et al. (Fri,) studied this question.