Biogenic synthesis of selenium nanoparticles from Nyctanthes arbor-tristis L. and evaluation of their antimicrobial, antioxidant and photocatalytic efficacy

Biogenic synthesis of nanoparticles has been established as an environmentally benign and sustainable approach. This study emphasizes biosynthesis of selenium nanoparticles (SeNPs) utilizing leaf extract of Nyctanthes arbor-tritis L., well known for its abundant bioactive compounds. Various analytical techniques were employed for characterization of synthesized SeNPs. X-ray diffraction (XRD) spectroscopy confirmed the crystalline structure and revealed the average crystalline size of SeNPs to be 44.57 nm. Additionally, UV–Vis spectroscopy confirmed successful synthesis of SeNPs by validating the surface plasmon resonance (SPR) properties of SeNPs. FTIR analysis data revealed different bonds and their corresponding functional groups responsible for the synthesis and stability of synthesized SeNPs. DLS and zeta analysis revealed that 116.5 nm sized SeNPs were stable in nature. Furthermore, field emission scanning electron microscopy (FE-SEM) validated the spherical morphology of SeNPs with a size range of 60–80 nm. Inductively coupled plasma-optical emission spectroscopy (ICP-OES) determined the concentration of SeNPs in the obtained colloidal solution. Antioxidant activity of synthesized SeNPs was evaluated employing DPPH and H2O2 assay, revealed that the synthesized SeNPs were effective antioxidant agent. Additionally, antimicrobial potential was evaluated against a panel of Gram-positive and Gram-negative bacteria and found to be effective at higher concentration of SeNPs. SeNPs also exhibited strong anti-biofilm activity while evaluated against various biofilm producing bacteria like Escherichia coli, Staphylococcus epidermidis and Klebsiella pneumonia. The cytotoxicity of the bio-synthesized SeNPs was evaluated against HEK 293 cell line, exhibited minimal toxicity even at concentration 100 μg/mL with 65% viable cells. SeNPs has also been evaluated for dye degradation which has indicated excellent photocatalytic activity of synthesized SeNPs. The experimental data obtained altogether demonstrated that synthesized SeNPs exhibited significant antimicrobial and anti-biofilm activity against various pathogens, and also showed significant antioxidant and photocatalytic efficiency.