Figure S17 from HDAC6 Inhibition Releases HR23B to Activate Proteasomes, Expand the Tumor Immunopeptidome and Amplify T-cell Antimyeloma Activity

Fig. S17. Effect of HDAC6 inhibitors on the expansion of MM spheroids cultured alone or with T-cells. MM patient CD138+ cells (50,000/ sample) were cultured in 50 μL of Matrigel (Corning) in 96-well plates according to the manufacturer’s protocol. Spheroids were allowed to form and maintained at 37 °C. Cells were then treated with HDAC6 inhibitors (1 uM) for 24 h followed by co-culture with T-cells (E:T 2;1) for another 24 h. Values represent the average of triplicate measurements.