Greenly engineered bimetallic Ag-ZnO nanohybrids for synergistic antibacterial enhancement

The escalating issue of infectious agents developing resistance to traditional antibiotics has spurred ongoing research into effective and broad-spectrum antimicrobial solutions. This study focuses on the fabrication of silver-zinc oxide Nanohybrids (A-ZnO-NHs) with varying silver content (1 %, 3 %, 5 %) using a simplified modified sol–gel method, further enhanced by a green synthesis approach utilizing orange peel extract for the generation of silver nanoparticles. The physicochemical characteristics of the A-ZnO-NCs were thoroughly examined. X-ray diffraction analysis verified the presence of Ag nanoparticles within the zinc oxide and scanning electron microscopy revealed the nanoscale silver particles uniformly distributed on the spherical zinc oxide nanoparticles. Transmission electron microscopy indicated that the Ag-ZnO-NCHs ranged in size from 10 to 20 nm. X-ray photoelectron spectroscopy analysis confirmed the formation of strong chemical bonds between the silver and zinc oxide surfaces in the nanohybrids. This study explored into the structural, morphological, and antimicrobial characteristics of the A-ZnO-NHs at different compositions. The bactericidal efficiency of the A-ZnO-NHs was assessed against both gram-positive and gram-negative bacterial strains. The impact of the A-ZnO-NHs on the cellular structure and chemical composition of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) was also explored. The findings revealed that the A-ZnO-NHs with 3 % silver content demonstrated higher antimicrobial activity against E. coli and S. aureus compared to other compositions and pure zinc oxide nanoparticles. The antimicrobial activity decreases when the concentration of silver increases because the silver particles agglomerate together, reducing their surface area and lessening their effectiveness as antibacterial agents, despite their potential for various applications.