ht-HiTE: High-Throughput DNA extraction from FFPE tissue v1

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Abstract

This protocols outlines a high-throughput method for DNA extraction from FFPE material. The workflow has been automated on a Biomek i5 platform. 1 to 2 tissue scrolls (10 µm) or tissue macrodissected from glass slides is used as input and eluted in 60 µl after nucleic acid purification. Mean and median yield is 86.6 and 72.0 ng/µl (equivalent to 4.3 to 5.3 µg of total DNA).

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Christoph Geisenberger (Fri,) studied this question.

synapsesocial.com/papers/68e78f53b6db643587700e03 https://doi.org/https://doi.org/10.17504/protocols.io.6qpvr3jr3vmk/v1

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4Abstract 5054: Optimization and evaluation of an FFPE dual extraction protocol for next-generation sequencing applications2024
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