Key points are not available for this paper at this time.
This protocol describes isolation of nuclei from fresh-frozen human knee cartilage for use in Omics analyses, including RNA-sequencing of ATAC-sequencing. Tissue dissociation was the most critical step in optimization which was monitored by nuclei yield, integrity and purity. Methods for quality control are also described.
Sakamoto et al. (Tue,) studied this question.