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CBX2 regulates immune transcriptional profiles. A, Volcano plot of differential gene expression based on shCBX2 vs. shCTRL in the PEO1 cell line. Immunoblot against CBX2 in PEO1 shCTRL and shCBX2 cells. Loading control, β-actin. B, Volcano plot of differential gene expression based on CBX2 KO vs. CTRL in the OVCAR4 cell line. Immunoblot against CBX2 in OVCAR4 CTRL and CBX2 KO. Loading control, β-actin. Asterisks indicate cell lines used for RNA-seq. C, Volcano plot of differential gene expression based on CBX2 OE vs. CTRL in the OVCAR cell line. Immunoblot against CBX2 in OVCAR4 control (CTRL) and CBX2 OE. Loading control, β-actin. D, Overlap of OVCAR4 CBX2 CRISPR knockout vs. OVCAR4 CBX2 OE. Inverse expression top hits: EMT and inflammatory response. E, Evaluation of hallmark pathways identified TNFα signaling via NF-κB inflammatory responses, and IL2 STAT5 signaling pathways. Direct relationship between CBX2 status and CXCL1 and CXCL8 (IL8) noted (black arrows).
Iwanaga et al. (Mon,) studied this question.