OT-2 Custom Mixing with Single or Multi-Channel Pipette v1

This protocol is created to help efficiently produce high-throughput plates with different reagents using either a single or multi-channel pipette controlled by an Opentrons OT-2 robot. By running this script, users can achieve a precise layout of reagents in specified volumes across the final plate(s) using a single-channel or multi-channel pipette based on a customizable Excel input file. The protocol uses a Python script for the Opentrons OT-2 robot in conjunction with an Excel file that contains all the necessary parameters and will be processed by the Python script. This Excel file contains customizable variables such as the number of final plates, transfer volumes, plate types, and the type of pipette used. This method ensures the accurate distribution of reagents based on user-defined configurations, making the high-throughput process more efficient. This protocol has been used in our laboratory to check how different quorum sensing systems work in P. putida KT2440. This protocol is a set of instructions or description of the LAP repository entry LAP-CustomReagentMixingMultiSinglePip-OT2-1.0.0 You can find the script and complementary information for this specific version of the protocol in this LAP entry link and GitHub Link to LAP entry documents