Figure 2 from Spatial Landscape of Malignant Pleural and Peritoneal Mesothelioma Tumor Immune Microenvironments

Interactions between immune cells and tumor cells in malignant mesothelioma. A, Heatmap depicting cell–cell contacts in patients with MPM or MPeM. Columns represent normalized pairwise cell–cell contact scores, and rows represent samples. B, Comparison of contact scores between tumor cells and the overall immune cell population and contact scores specifically involving tumor cells and CD4+ T cells, CD8+ T cells, macrophages, and Tregs between cohorts of patients with MPM and MPeM. Statistical analysis was performed using FDR-adjusted one-sided multiple Mann–Whitney U tests. Each dot represents the cell contact score of an individual core. C, Representative cases from two samples with MPM and two patients with MPeM demonstrating mIF, H&E staining, and cell-type analyses. Images were captured at 10× magnification. Scale bar, 100 μm. D, Heatmap depicting types of CNs (rows) and relative enrichment above or below the mean across neighborhoods for B cells (CD20+), CD8+ T cells, CD4+ T cells, Tregs (FOXP3+), tumor cells (Pan-CK+), and macrophages (CD68+). Likelihood of enrichment was calculated as log odds ratio values normalized between 5 and −5. E, Pie charts representing median relative contributions of CNs in MPM (top) and MPeM (bottom) biologically independent samples.