Isolation of Lactobacillus Species from Different Types of Milk and Evaluate their Antagonistic Activity Against Pathogens

Animal milk has been consumed by humans for a very long time. In the food industry, the probiotic Lactobacillus is regarded as a good substitute to lower the risk of food-borne illnesses. The bacteria Lactobacillus aids in the body’s nutrient absorption, digestion, and defense against pathogens. Determination of Lactobacillus from unfermented and reconstituted Milk. The initial and biochemical tests allowed for the identification of the organisms. The two pathogens, identified as Escherichia coli and Staphylococcus aureus were isolated in light of the findings. The purpose of these two pathogens, selection was antagonistic research. With the cross-stretched method, the antagonistic activity test was conducted. The outcome demonstrates that there is more inhibition displayed by the Lactobacillus grown in buffalo milk. In an antagonistic test, the probiotic organism inhibits the test pathogens.