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Abstract This work aimed to examine the impacts of dietary Bacillus subtilis (BS) supplementation on several parameters. Twelve groups of six hundred-day-old male Ross chicks were created for the study. Each treatment was allotted into five replicates, with ten birds each. Four distinct diet treatments were provided for 42 days: diet A served as the control, whereas diets B, C, and D had 125, 250, and 500 mg of BS per kg of feed. Every diet was fed in three stages: continuous (days 1-42) and starter only (days 1–21), finisher only (days 22–42), and both. The results showed that the D-3 and C-3 groups had significantly higher feed intake (FI), body weight gain (BWG), feed conversion ratio (FCR), livability, and European Production Efficiency Factor (EPEF).juo09 The D-3 and C-3 groups also showed the largest relative weights of the liver, gizzard, pancreas, bursa, and spleen, as well as the enhanced weights of these organs. Groups D-3 and C-3 had high villus height, decreased crypt depth, and enhanced VH: CD in the ileum, jejunum, and duodenum. The high levels of BS (D-3 and C-3) eliminated Salmonella , C. perfringens , and E. coli from the ileum, caecum, and colon. From the results above, a conclusion could be drawn that dietary BS supplementation could be a safe substitute for AGPs in broiler diets.
Khan et al. (Sat,) studied this question.