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Abstract Background Thrombocytopenia, common in preterm newborns, may increase bleeding risk and is often treated with transfusions. Recent studies reveal that transfusing platelets at a high threshold worsens outcomes, possibly due to a “developmental mismatch” between adult‐derived platelets and neonatal hemostatic system. Cord blood‐derived platelet concentrates (CBPCs) could be an alternative for newborns. Our study aims to produce and evaluate the quality parameters of CBPCs during storage. Study Design and Methods Cord blood was collected from placentas after near‐term and full‐term pregnancies. Several production methods were attempted to obtain CBPCs, varying centrifugation settings, filtration, and dilution procedures. Adult‐derived platelet concentrates (PCs) processed with the same methods, and standard PCs from five buffy‐coats were used as controls. Storage tests were performed on days 2, 4–5, 7 from the collection. Results CBPCs parameters were compared with adult‐derived PCs, and no significant differences were found for mean platelet volume (MPV), swirling, morphology, glucose, lactate, pCO 2 , and pO 2 . pH and bicarbonate were lower in CBPCs. Some significant differences between methods in CD62P expression and JC‐1 ratio were observed. Compared with standard PCs, CBPCs showed lower platelet concentration, pH, and JC‐1. Additionally, both in CBPCs as well as in control PCs, the apoptosis marker phosphatidylserine was elevated. Discussion CBPCs were of comparable quality to control PCs during storage. However, apoptosis markers in both groups were elevated, suggesting processing and storage of low volumes of PCs require further optimization. Also, filtration of low volumes leads to significant platelet loss, an issue that requires remedy.
Cortesi et al. (Fri,) studied this question.