Analysis of Pesticide Residue in Fruit Using the QuEChERS Method Coupled With LC-MS/MS Detection

Identifying pesticide residues in fruits is essential to both regulatory compliance and food safety. For this use, liquid chromatography combined with tandem mass spectrometry, or liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), is an advanced analytical method that provides excellent sensitivity and specificity. This work offers a thorough procedure for employing LC-MS/MS to extract, clean up, and quantify various pesticide residues in fruit samples.The QuEChERS technique, which uses acetonitrile and a salting-out step for effective partitioning, first homogenizes the fruit samples before extracting them. Dispersive solid-phase extraction (d-SPE) then eliminates matrix interferences from the extract. I went to the Sardar market in Surat and picked some fruits, such as watermelons, oranges, and grapes. Next, the homogenised extract was made. LC-MS/MS uses liquid chromatography to separate the pesticides from the cleaned extract, while tandem mass spectrometry uses precursor ion fragmentation and product ion detection to provide thorough mass analysis. Calibration curves built using established pesticide standards enable accurate quantification. This technique shows great performance in simultaneously detecting trace amounts of many pesticides, ensuring the robustness and dependability required for food safety evaluations. Pesticide residue analysis using LC-MS/MS guarantees adherence to strict regulatory limitations, protecting consumer health in the process.