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In the context of the ERC Starting Grant HoloE2Plant N° 101039541 “Exploring the Holobiont concept through a Plant Evolutionary Experiment study”, long read bacterial and fungal reference genomes were performed with PacBio Sequel II technology. The purpose of these genomes is to help in the Synthetic Microbial Communities (SynComs) construction. As DNA extraction is a crucial step prior to obtain high-quality genomes, we developed a two-days DNA extraction protocol efficient for the extraction of High-Molecular-Weight (HMW) genomic DNA on both gram-positive/negative bacteria and fungi. To our knowledge, this is the first protocol enabling to extract HMW DNA from both bacteria and fungi. Our protocol was adapted from the four protocols provided in the references.
Norest et al. (Fri,) studied this question.