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The growth of bivoltine silk production in eastern and north-eastern india lags behind drastically in comparison to southern states due to non-availability of suitable bivoltine hybrids. The major constraints in bivoltine popularization are adverse climatic conditions. to overcome this limitation and to continuously rear bivoltine hybrids in these harsh climatic conditions throughout the year, the present proposal aims to identify robust and abiotic stress tolerant bivoltine silkworm breeds through integrating conventional breeding (directional selection) for improved productivity and advanced genome technologies such as MAS (marker assisted selection), gene specific markers for high humidity and thermo-tolerant DNA markers (Pyx3, Pyx4, S0803 and S0816) for tolerance to high temperature and high humidity and other abiotic factors. These robust hybrids could be reared throughout the year and would provide opportunities for further popularizing and improving bivoltine raw silk production in the eastern and north-eastern region. the present investigation identified wb1hh bivoltine breed to be having highest survival rate and better shell ratio of 18.81% in abiotic stress condition. MAS selection identified DNA markers in all breeds and among them WB1HH showed all four markers, confirming the genetic ability of the breed for tolerance to high temperature and humidity.
VS et al. (Sun,) studied this question.