Supplementary Figure S2 from Early Detection of Malignant and Premalignant Peripheral Nerve Tumors Using Cell-Free DNA Fragmentomics

Supplemental Figure 2. Diminishing improvements in accuracy with deeper sequencing. MPNST samples sequenced to ∼25x were downsampled in five iterations per goal coverage (Methods). Size selected, copy number derived tumor fraction variability was noted between iterations less than or equal to 3x with statistically significant differences in tumor fraction (*, Welch’s T-test) between 25x coverage and 3x or below sequencing depths. No statistical difference (ns) was noted in tumor fraction between 25x coverage and sequencing depths greater than or equal to 6x.