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In the current investigation, the genetic relations, and the genetic dimension of seven olive varieties (Olea europaea L.) were identified using, ISSR, and SSR markers. The study involved uses leaves from different samples for DNA extraction. The DNA amount ranged from 150 to 400 micrograms with purity ranging between 1.6 to 1.9. Agarose gel electrophoresis is applied to evaluate PCR reaction success. The ISSR marker, which used 4 primers, showed different results for the multiplication. Various bands were observed that differed from each other, resulting in a total of 93 bands - 2 general and 91 different. The primer also distinguished unique bands, with 4 unique bands and 7 absent bands. The primer with the highest molecular size (1500bp) was UBC-817, while the lowest molecular volume (200bp) was observed in the UBC-826 primer. The marker results indicated that the two types, Suranie (3) and Frantoio (4), had the lowest hereditary dimension of (0.030). The Frantoio and Santacatrina varieties showed the highest genetic difference, with a value of 0.622. According to the genetic relationship analysis, there were three main groups. The first group consisted of a single category (5), while There were two subgroups in the second group, B1 and B2. Subgroup B1 had only one category, while the rest of the categories were in Subgroup B2.. Even though these markers use different mechanisms to detect variance and genome coverage, they complement each other. Moreover, five SSR markers were used in this study to describe seven olive cultivars and analyze their genetic relationship. Four out of five primers showed positive results, while one was not present. The SSR markers were effective in identifying the similarity of collected species, as they are specialized indicators ISSR markers.
Al.obeide et al. (Thu,) studied this question.