Recombinant stabilised poliovirus virus-like particles produced in multiple expression systems demonstrated equivalent or superior immunogenicity compared to the current inactivated polio vaccine in animal models.
Recombinant stabilised poliovirus VLPs produced in various expression systems demonstrate equivalent or superior immunogenicity and thermostability compared to current inactivated polio vaccines, offering a viable genome-free vaccine candidate.
Polioviruses have caused crippling disease in humans for centuries, prior to the successful development of vaccines in the mid-1900's, which dramatically reduced disease prevalence. Continued use of these vaccines, however, threatens ultimate disease eradication and achievement of a polio-free world. Virus-like particles (VLPs) that lack a viral genome represent a safer potential vaccine, although they require particle stabilization. Using our previously established genetic techniques to stabilize the structural capsid proteins, we demonstrate production of poliovirus VLPs of all three serotypes, from four different recombinant expression systems. We compare the antigenicity, thermostability and immunogenicity of these stabilized VLPs against the current inactivated polio vaccine, demonstrating equivalent or superior immunogenicity in female Wistar rats. Structural analyses of these recombinant VLPs provide a rational understanding of the stabilizing mutations and the role of potential excipients. Collectively, we have established these poliovirus stabilized VLPs as viable next-generation vaccine candidates for the future.
Sherry et al. (Sat,) conducted a other in Poliovirus vaccination (preclinical). Recombinant stabilised virus-like particles (rsVLPs) vs. Inactivated polio vaccine (IPV) was evaluated on Neutralising antibody response and protection against virus challenge. Recombinant stabilised poliovirus virus-like particles produced in multiple expression systems demonstrated equivalent or superior immunogenicity compared to the current inactivated polio vaccine in animal models.