Neonatal mice orally inoculated with equine rotavirus A strains G3P[12] and G14P[12] developed short-lived diarrhea at rates of 88% and 61%, respectively, along with pronounced intestinal histological alterations.
What are the clinical, virological, and pathological features of equine, porcine, and bovine rotavirus A infection in neonatal mice?
Neonatal mice can serve as a preclinical model for studying the pathogenesis and vaccine efficacy of equine, porcine, and bovine rotavirus A strains.
Tasa de eventos absoluta: 88% vs 0%
Group A rotavirus (RVA) infections are a leading cause of neonatal diarrhoea in foals. Neonatal mice could serve as a useful tool to study the pathogenesis of equine RVA (ERVA) as well as a preclinical model for assessment of vaccine efficacy. This study aimed to comparatively evaluate the clinical, virological and pathological features of ERVA G3P12 and G14P12 infection in neonatal mice and compare them with porcine OSU G5P7 and bovine UK G6P5 RVA reference strains. Neonatal mice orally inoculated with equine, bovine and porcine RVA developed short-lived diarrhoea at variable rates, G14P12 (61%) and G3P12 (88%). Viral replication kinetics for all strains were characterized by a gradual decline in viral load to levels below the limit of detection by 72–96 h post-infection (hpi), in line with the reduction in the number of infected enterocytes demonstrated via RNAscope ® in situ hybridization. Importantly, the clinical and viral replication kinetics correlated with significant microscopic intestinal alterations characterized by enterocyte vacuolation, scalloping and hyperplasia with a peak occurring at 48 hpi and persisting until at least 96 hpi. Overall, neonatal mice develop a disease phenotype of short duration following infection with equine, porcine and bovine RVA strains characterized by diarrhoea and pronounced histological alterations in the intestinal villi. The limited intestinal viral replication is likely associated with host restriction. The clinical and pathological phenotypes developed by neonatal mice following experimental infection could serve as a preclinical tool to assess vaccine efficacy and for pathogenesis studies involving RVA of equine, porcine and bovine origin.
Gamage et al. (Thu,) conducted a other in Rotavirus A infection (n=138). Equine rotavirus A (ERVA) G3P[12] and G14P[12] vs. Mock-infected was evaluated on Incidence of diarrhea. Neonatal mice orally inoculated with equine rotavirus A strains G3P[12] and G14P[12] developed short-lived diarrhea at rates of 88% and 61%, respectively, along with pronounced intestinal histological alterations.