Living myocardial slices retained patient-specific functional properties, exhibiting lower intrapatient versus interpatient variability and reflecting distinct etiologies and therapeutic histories.
Observational (n=138)
Background: findings correlate with clinical characteristics is unknown. This study aimed to evaluate whether LMS retain patient-specific functional and pathological characteristics, reflecting diverse etiologies, pharmacological regimens, and clinical interventions. Methods: = 138). Functional assessment of freshly prepared LMS included refractory period, stimulation threshold, force-frequency relationship, post pause potentiation, contractile force, alongside simultaneous optical recordings of calcium transients and action potentials. Variability and grouping analyses were conducted to identify features linked to patient-specific parameters, such as etiology and therapeutic history, including prior left ventricular assist device (LVAD) implantation and amiodarone usage. Results: LMS exhibited lower intrapatient variability (LMS from the same patient) compared to interpatient variability (LMS from different patients), confirming their ability to retain patient-specific functional properties. LMS from LVAD-treated patients exhibited reduced intrapatient variability and reduced diastolic tension, correlated with lower N-terminal pro-B-type natriuretic peptide levels. Stratification by etiology revealed distinct functional characteristics, including enhanced contractile force in titin-mutant LMS and a positive force-frequency relationship in ischemic cardiomyopathy-derived LMS. LMS derived from amiodarone-treated patients demonstrated prolonged action potential duration, reduced excitability at higher pacing frequencies, and enhanced post pause potentiation, reflecting the drug's established pharmacological effects. Conclusions: LMS effectively capture distinct functional parameters associated with patient-specific features. These findings establish LMS as a valuable translational platform for personalized cardiac research, therapeutic testing, and precision medicine.
Geest et al. (Tue,) conducted a observational in Cardiac disease (n=138). Living myocardial slices (LMS) assessment vs. Interpatient vs intrapatient variability was evaluated on Retention of patient-specific functional and pathological characteristics. Living myocardial slices retained patient-specific functional properties, exhibiting lower intrapatient versus interpatient variability and reflecting distinct etiologies and therapeutic histories.