Porcine Organoid-Derived Monolayers in Static and Dynamic Platforms: Toward a Biomimetic In Vitro Intestinal Model

Key Points

• Dynamic systems promote earlier barrier maturation in porcine intestinal organoids, improving physiological relevance.
• Epithelial differentiation and stable permeability were enhanced in dynamic conditions, indicating better model fidelity.
• Applications of organ-on-chip platforms reduce the necessity for animal models in studying intestinal function.
• The findings highlight the potential of biomimetic in vitro models in gastrointestinal research and drug testing.