Nanoceria has been studies for many decades given its capability in quenching reactive oxygen species (ROS) in inflammatory diseases. The regenerative exchanges between Ce3+ and Ce4+ as well as the ratio between these two cations have been reported as one of the most important factors for enhancing their ROS scavenging activity. In this study, a novel method for increasing the active sites and the ratio of Ce3+/Ce4+ utilising layered double hydroxides (LDH) nano system has been developed. Ce doped LDH nanoparticles with various Ce-loading amounts were prepared, and the physicochemical properties were characterised. The anti-hydrogen peroxide (H2O2) capability of these Ce-LDH NPs has been evaluated in vitro against the treated RAW 264.7 macrophages. The optimal Ce-LDH NPs were then introduced into a 3D cell-hydrogel construct resembling the pathological morphology of atherosclerosis to investigate their ability to capture ROS in a more complex cell system. The successful development of this Ce-doped LDH nanosystem provides a promising biocompatible agent for future treatment of inflammatory disorders.
Nguyen et al. (Wed,) studied this question.