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This is a In Vitro Study study (also classified as: Observational).

August 19, 2025Open Access

Histone-driven hypercoagulation contributes to the lethal triad of acute trauma-induced coagulopathy

CBChristian BraunUniversity of Lucerne MMMarco MannesUniversität Ulm DSDoreen Tabea SpiegelburgUniversität Ulm

Key Points

Hypercoagulation was significantly driven by histones, a condition likely exacerbating patient mortality from trauma.
Both acidosis and hypothermia independently triggered increased platelet activation and consumption, leading to clot formation.
Using an ex vivo human whole blood model, the study assessed platelet response under varying trauma conditions.
The findings suggest targeting histones may provide a novel therapeutic avenue to address trauma-induced coagulopathy.

Abstract

Severe tissue injury and hemorrhagic shock can result in trauma-induced coagulopathy (TIC), acidosis, and hypothermia, denoted as lethal triad. This condition exacerbates trauma complications and contributes to organ dysfunction and mortality, prompting the need to better define its complex pathomechanisms in the blood. Therefore, we developed a standardized ex vivo human whole blood (hWB) model to differentiate the impact of various pathophysiological conditions, damage-associated molecular patterns (DAMPs), and key inflammatory mediators on TIC development. Modelling incremental grades of the lethal triad, we assessed the resulting thrombo-coagulopathy by monitoring platelet activation, clot formation, and mediator release. Both acidosis and hypothermia independently triggered platelet activation (with enhanced surface CD62P/CD63 expression) and platelet consumption, leading to ex vivo clot formation. Completing the lethal triad with additional dilutional coagulopathy revealed even more pronounced effects. Further dissecting underlying cross-talk mechanisms, platelet-rich plasma (PRP) or isolated platelets exposed to similar conditions exhibited no relevant impact on platelet function. Notably, histones-unlike HMGB1, pro-inflammatory cytokines or anaphylatoxins-amplified the lethal triad-driven hypercoagulative TIC response in both hWB and PRP. In conclusion, our findings in a modular, clinically relevant whole blood model highlight histones as a central driver of TIC. Furthermore, tracking early TIC progression in this model provides an effective and efficient platform for evaluating novel therapeutic approaches to target drivers of coagulopathy.

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