Accurate measurement of Valsartan and Sacubitril in pharmaceutical formulations is crucial for ensuring both therapeutic efficacy and safety in heart failure treatment. This review compares three analytical techniques—Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), UV spectrophotometry, and LC-MS/MS—aiming to identify the most effective method for drug quantification. RP-HPLC emerges as the most suitable method for routine analysis due to its superior resolution, precision, and reproducibility. In contrast, UV spectrophotometry serves as a simpler, cost-effective solution for bulk drug testing in large batches, offering reduced complexity but with less sensitivity. Advanced techniques such as LC-MS/MS and spectrofluorimetric analysis deliver exceptional sensitivity, making them ideal for pharmacokinetic studies; however, they are less practical for everyday testing. Furthermore, green chemistry initiatives are gaining traction as they minimize environmental impact while maintaining high analytical accuracy. Although RP-HPLC remains the preferred method, the evolving landscape of analytical techniques, coupled with sustainable practices, is reshaping the future of pharmaceutical quality control.
Madhav et al. (Sun,) studied this question.