Internal Cycling Influences Nutrient Changes Leading to Altered Nutrient Limitation in Eutrophic Lake

Lake eutrophication is governed by persistent anthropogenic nutrient inputs, primarily nitrogen (N), phosphorus (P) and cryptic internal nutrient cycling processes that sustain bioavailable nutrient pools. While the impact of external nutrient loads on lake eutrophication has been extensively studied, the role of internal nutrient cycling in lake ecosystems remains underexplored. In this study, the hierarchical bootstrap generalized linear model (HBGLM) to long-term summer water quality data (1999–2020) from Lake Dianchi, China, to explore the relative importance of nitrogen (N), phosphorus (P), as well as the limitations of N and P on the growth of phytoplankton. The results revealed that from 1999 to 2020, the Chla and TP concentrations decreased by 49% and 78%, respectively, and that internal nutrient cycling significantly influenced changes in nutrient concentrations, reflecting the relationships among N, P, and chlorophyll a (Chla). Particularly in 2007, 2013, and 2017, the long-term trends of the TN:TP ratio, an indicator of potential nutrient limitation in the lake, were consistent with changes in the distributions of the average slopes of TN and TP across different periods, indicating that these years primarily exhibited patterns of colimitation by N and P or P limitation, indirectly confirming that Lake Dianchi will transition from N and P colimitation to being limited primarily by P. This study reveals that N is typically the primary limiting element, while P is a key element promoting water eutrophication. To further validate improvements to existing eutrophication mitigation models, conducting carefully de-signed experiments at different scales is recommended.