Sample processing flow chart. LC, library construction; QC, quality control; SEQ, sequencing. aData from these samples are not included in this study. bPlasma sample was not available for testing with LBx. cMatching plasma comes from clinical trial samples. dTested externally by the Qiagen Therascreen BRAF V600E RGQ PCR Kit. eCommercially procured tissue-matched plasma samples that were BRAF wild-type by tissue testing and were used for concordance testing.
Yaeger et al. (Tue,) studied this question.