September 17, 2025

Validation of Pregnancy Associated Glycoprotein Based Rapid Diagnosis Kit with the Aid of Ultrasonography in Cattle and Buffaloes

Key Points

The Prompt BovEasy® Pregnancy Rapid Test Kit demonstrated accuracy rates of 97.96%, 99.33%, and 99.43% on days 28, 32, and 36 after insemination.
Validation involved 141 dairy animals, confirming the reliability and specificity of the PAG based test kits for early pregnancy diagnosis.
The incidence of false positive and false negative results was low, highlighting the kits' effectiveness in practical farm settings.
This method can optimize herd management and improve productivity through timely pregnancy detection in dairy cattle.

Abstract

Early pregnancy diagnosis in the dairy cattle is always challenging, which makes the dairy as profitable by timely conception and timely calving procedures. Various methods are available for diagnosing pregnancy in cows, including estrus observation, rectal palpation, and ultrasonography, which are commonly employed examination techniques. The present study was conducted @ the Veterinary College, Shivamogga, Karnataka, by using Pregnancy Associated Glycoprotein (PAG) based test kits as a reliable method and was validated in 141 dairy animals. The overall accuracy of the Prompt BovEasy® Pregnancy Rapid Test Kit was to the extent of 97.96, 99.33 and 99.43 % & on days 28,32, and 36 respectively which revealed these kits can be used for early detection of pregnancy from day 28 onwards and could be easily integrated into farm routines, with proven specificity and accuracy. The incidence of false positive and false negative was low. To optimize herd management and productivity, this kit could be used to early detection of early pregnancy in dairy animals with accuracy.

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