The extraction of DNA from biological samples precedes many research and commercial applications. This study compares surface treatments of cellulose (a low-cost binding matrix) to enhance binding and elution of DNA to paper-based dipsticks. Cellulose paper was modified with poly-L-lysine, silica, or guanidine, as well as subjected to TEMPO-based oxidation. Subsequently, binding and elution behaviour of fragmented salmon sperm DNA to dipsticks was evaluated. Qubit fluorimetry and agarose gel electrophoresis measurements indicated that TEMPO-based oxidation significantly increased the binding of DNA and its elution from dipsticks, while silica modifications bound DNA efficiently, but strongly retained it. Leaching of select modifiers (guanidine, silica and poly-L-lysine) was indicated by UV/Vis spectroscopy, indicating that further optimization of attachment processes is required. This study is the first to compare multiple cellulose surface treatments for their influence on DNA binding and elution, especially the use of TEMPO-based oxidation for this purpose, and highlights some means of identifying leaching of modifiers during DNA capture at these surfaces and subsequent elution. While TEMPO-based oxidation proves a promising treatment to enhance DNA elution, further refinement of the approach is needed to ensure compatibility with molecular biology techniques.
Rutherford et al. (Thu,) studied this question.
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