September 19, 2025

Endophytic Diaporthe species from Brazil

Key Points

The study identifies several endophytic Diaporthe species from Brazil with a detailed phylogenetic framework.
Phylogenetic analyses utilize maximum likelihood methods on gene regions like tub2 and ITS, illustrating their evolutionary relationships.
Data includes sequence information such as GenBank accession numbers and PCR protocols for reproducibility.
The findings advance understanding of the biodiversity and ecological roles of Diaporthe species in their environments.

Abstract

Fig. S1. Maximum Likelihood (ML) phylogenetic tree of the tub2 sequences (big tree). The species/isolates obtained in this study are in bold. Ex-type strains are marked with an asterisk (*). Confidence values for ML-BS ≥ 70 % (RAxML) and BPP ≥ 0.95 are included near the nodes and the “-” indicates statistical support below the threshold values. The scale bar represents the expected number of changes per site. The tree was rooted to Diaporthella corylina (CBS 121124).Fig. S2. Maximum Likelihood (ML) phylogenetic tree of the representative tub2 sequences (small tree). The species/isolates obtained in this study are in bold. Ex-type strains are marked with an asterisk (*). Confidence values for ML-BS ≥ 70 % (RAxML) and BPP ≥ 0.95 are included near the nodes and the “-” indicates statistical support below the threshold values. The scale bar represents the expected number of changes per site. The tree was rooted to Diaporthella corylina (CBS 121124).Fig. S3. Maximum Likelihood (ML) phylogenetic tree of the representative ITS sequences. The species/isolates obtained in this study are in bold. Ex-type strains are marked with an asterisk (*). Confidence values for ML-BS ≥ 70 % (RAxML) and BPP ≥ 0.95 are included near the nodes and the “-” indicates statistical support below the threshold values. The scale bar represents the expected number of changes per site. The tree was rooted to Diaporthella corylina (CBS 121124).Fig. S4. Maximum Likelihood (ML) phylogenetic tree of the representative tef1 sequences. The species/isolates obtained in this study are in bold. Ex-type strains are marked with an asterisk (*). Confidence values for ML-BS ≥ 70 % (RAxML) and BPP ≥ 0.95 are included near the nodes and the “-” indicates statistical support below the threshold values. The scale bar represents the expected number of changes per site. The tree was rooted to Diaporthella corylina (CBS 121124).Fig. S5. Maximum Likelihood (ML) phylogenetic tree of the representative cmdA sequences. The species/isolates obtained in this study are in bold. Ex-type strains are marked with an asterisk (*). Confidence values for ML-BS ≥ 70 % (RAxML) and BPP ≥ 0.95 are included near the nodes and the “-” indicates statistical support below the threshold values. The scale bar represents the expected number of changes per site. The tree was rooted to Diaporthella corylina (CBS 121124).Fig. S6. Maximum Likelihood (ML) phylogenetic tree of the representative his3 sequences. The species/isolates obtained in this study are in bold. Ex-type strains are marked with an asterisk (*). Confidence values for ML-BS ≥ 70 % (RAxML) and BPP ≥ 0.95 are included near the nodes and the “-” indicates statistical support below the threshold values. The scale bar represents the expected number of changes per site. The tree was rooted to Diaporthella corylina (CBS 121124).Table S1. Gene regions used in this study, respective primer pairs, PCR protocols, and references.Table S2. GenBank accession numbers of sequences of Diaporthe species used in the phylogenetic analyses. Isolates and sequences obtained in this study are given in bold

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