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This is a Experimental Study study.

September 19, 2025Open Access

The effect of a special adjuvant with FSH on blood FSH level, superstimulation and embryo quality in dairy cattle

Key Points

Single injection of FSH dissolved in Montanide achieves comparable plasma FSH levels and superovulation results.
Both control and experimental groups showed similar embryo quality during assessments after insemination.
No adverse effects were reported at injection sites across all groups during the trial.
The traditional protocol's effectiveness was matched by the new approach using fewer FSH applications.

Abstract

Abstract Follicle-stimulating hormone (FSH) must be applied at 12-h intervals over 4–5 days in the traditional cattle superovulation protocol, which still needs to be improved. This research paper evaluated the superovulation results obtained by a traditional protocol or by a single administration of FSH dissolved in Montanide TM ISA-206 VG (MonISA-206). Control cows were superovulated with 10 mL of FSH (500 µg pFSH + 100 µg pLH) from day 7 to day 10 (for 4 days, twice daily i.m. injections, decreasing doses). Cows in the EG10 and EG7.5 groups were injected i.m. with 20 mL (100%, 10 mL + 10 mL) or 15 mL (75%, 7.5 mL + 7.5 mL) of the FSH and MonISA-206 mixture at once on day 7. All cows were inseminated 12 and 24 h after oestrus onset. The cows presented no pathology at the injection sites. Plasma FSH levels differed between the groups, but the interaction between hour and group × time was not different. Superstimulation and embryo quality results were similar between the groups. A single injection of FSH (both 100% and 75% doses) dissolved in MonISA-206 led to adequate plasma FSH levels and similar superovulation results to traditional FSH treatment, and caused no pathology at the injection sites.

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