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This is a Cohort Study study (also classified as: Quantitative Study, Experimental Study).

September 23, 2025

Melatonin Mitigates Abamectin‐Induced Subacute Hematotoxicity and Hepato‐Renal Toxicity in Rats by Regulating Oxidative Stress, Inflammatory Responses, and Apoptosis

HKHatice KarabodukGazi Hastanesi ÇAÇağlar AdıgüzelGazi Hastanesi MUMeltem UzunhisarcıklıGazi Hastanesi

Key Points

Melatonin reduces abamectin-induced hematotoxicity and hepato-renal toxicity in rats, improving organ health and blood parameters.
Significant increases in oxidative stress markers were observed in abamectin-treated rats, whereas melatonin alleviated these adverse effects.
Biochemical, immunohistochemical, and histopathological assessments showed that melatonin supplementation improved liver and kidney function.
Findings indicate that melatonin's anti-inflammatory and antioxidant properties can mitigate organ damage caused by pesticide exposure.

Abstract

Abamectin is a widely used pesticide due to its strong anthelmintic and insecticidal activity and has toxic effects on nontarget organisms. In this study, melatonin (10 mg/kg body weight), which has potent anti-inflammatory, antiapoptotic, and antioxidant effects against subacute hepato-renal toxicity of abamectin (0.5 mg/kg body weight) in rats, was evaluated for its potential to alleviate organ damage by biochemical, oxidative stress, immunohistochemical, histopathological, and cytopathological studies. In hepato-renal tissues of abamectin-treated rats, MDA and 8-OHdG levels, total oxidant status, and oxidative stress index significantly increased. In contrast, endogenous antioxidant enzyme activities and total antioxidant status decreased significantly. A significant decrease in acetylcholinesterase activity, TNF-α and caspase-3 immunopositive cells, and interleukin-17 levels were also detected in the tissues. Abamectin caused a substantial reduction in red blood cell, hemoglobin, hematocrit, and platelet counts and significantly increased white blood cell count. In addition, abamectin increased the activities of ALT, AST, ALP, and LDH, and the levels of total cholesterol, triglyceride, creatinine, uric acid, urea, and BUN, which are evaluated as biomarkers of blood hepato-renal tissues. At the same time, it caused a decrease in the levels of total protein and albumin. In addition, these changing biochemical parameters are accompanied by cytopathological and histopathological changes in hepato-renal tissues. However, exogenous melatonin supplementation reduced the oxidative stress caused by abamectin in rats and caused the pathological changes in hepato-renal tissues to be observed more mildly. It also reversed the changes in blood parameters and hepato-renal markers. In conclusion, this study suggests that exogenous melatonin supplementation may help significantly ameliorate abamectin-induced hepato-renal injury in rats through anti-inflammatory, antioxidant, and antiapoptotic mechanisms.

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