The main purpose of this article is to increase the effectiveness of bio-catalysts in technological environments to enhance technical capabilities. With an ambition to explore the developments made in computational techniques, this paper presents a methodology for transforming the characteristics of enzymes and create enhance bio-catalysts. The authors of this study review various approaches of enzyme engineering, such as aided evolution and rational design. The puts more emphasis on the strategic application of intermolecular collaboration, which enhance the attraction between substrates and enzymes. The research also reviews the challenges and development made in approaches employed in immobilizing enzymes. These approaches include a technique for repairing objects by employing polyketone polymer and assesses traditional approaches of permanently attaching objects together. Towards the end of the research, authors introduce a technique for immobilization, such as Huisgen 1, 3-dipolar cycloaddition, and Staudinger ligation. In addition, the research assesses the applications of nanoparticles in nano-biocatalysts and the integration of green chemistry standards and principles in enzyme bio-catalysts.
S. Kim (Thu,) studied this question.