What type of study is this?

This is a Pre-Registered Trial study.

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September 28, 2025

Sanger Tree of Life Fragmented DNA clean up: Automated SPRI v2

Key Points

The automated protocol maintains high effectiveness for sheared DNA across various taxonomic groups.
Output consists of DNA suitable for submissions to PacBio Low Input or Ultra Low Input long read sequencing.
The method utilizes automated SPRI for higher throughput, enhancing efficiency compared to manual techniques.
This process was adapted from an existing manual cleanup protocol, reflecting advancements in DNA handling.

Abstract

This protocol describes the automated clean up and shorter fragment removal from fragmented DNA following the Sanger Tree of Life HMW DNA Fragmentation protocols, using PacBio AMPure PB beads and the Thermo Fisher KingFisher™ Apex. This process is highly effective for sheared DNA from all of the taxonomic groups covered by the Tree of Life Programme. The output of this protocol is DNA which can be submitted for long read sequencing, including PacBio Low Input (LI) or Ultra Low Input (ULI) sequencing. This protocol was adapted from Sanger Tree of Life Fragmented DNA clean up: Manual SPRI to include automation for a higher throughput of samples. Acronyms HMW: high molecular weight SPRI: solid-phase reversible immobilisation LI: low input ULI: ultra-low input

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