The main objective of this study is to investigate the chemical composition, antioxidant and antimicrobial activities of the essential oil of Artemisia herba-alba Asso. of southern Algeria. Essential oil of Artemisia herba-alba Asso was extracted by hydrodistillation, and their chemical composition was identified by GC/MS Antioxidant activity of essential oil, has been done by using DPPH assay. The antimicrobial activity of essential oil was realised by the agar disc diffusion method. The essential oil extracted from the aerial parts by hydrodistillation was analysed by GC/MS. 39 constituents, representing 99.3% of the oil, were identified, of which the major ones, Thujone (12,759 %), Camphor (7,751%), Eacalyptol (4,525%), Isoborneol (1,119%). IC50 values observed for DPPH essay were 20,27 ±0,767 mg/ml. On the other hand, this oil was found effective against all tested strains; this activity ranged from 15.67±1.53 mm with Listeria innocua CIP 74915. These results provided evidence that the studied plant might indeed be a potential source of natural antioxidant and antimicrobial agents.
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