Elevated fibrinogen (OR 2.662), male gender, and elevated lipoprotein(a) were identified as independent risk factors for premature coronary artery disease, alongside 440 differentially expressed proteins.
Case-Control (n=129)
No
What are the key differential proteins, biological pathways, and independent risk factors associated with premature coronary artery disease?
Proteomic analysis reveals that ribosomal and translational proteins, along with clinical risk factors like male sex, fibrinogen, and lipoprotein(a), are significantly associated with premature coronary artery disease.
Odds Ratio: 2.662 (95% CI 1.471–4.818)
p-value: p=0.001
BACKGROUND: Premature coronary artery disease (PCAD) is characterized by early onset, rapid progression, and poor prognosis, which seriously affects patients' health and quality of life. In this study, we analyzed the proteomic network and biological pathways of PCAD patients by bioinformatics methods, and mined out the key differential proteins, which provided a theoretical basis for clinical intervention. METHODS: Patients who attended the heart center of the First Affiliated Hospital of Xinjiang Medical University from January 2023 to December 2024 and completed coronary angiography were selected. According to the relevant inclusion and exclusion criteria, a total of 129 patients were included, including 69 in the PCAD group and 60 in the control group. The clinical baseline data of the patients were systematically analyzed. Plasma protein extraction, trypsin digestion and mass spectrometry were completed. The mass spectrometry data were initially separated with the help of proteomics software, and the differential proteins were functionally enriched by RStudio software. Protein interaction networks were constructed by STRING platform and core differential proteins screened were visualized using Cytoscape software (MCODE plug-in). RESULTS: Differences in gender, smoking, alcohol consumption, hypertension, diabetes, HDL-C, Glu, FIB, LPa, NT-pro-BNP, PCT, and IL-6 were statistically significant (P < 0.05). Sex (P = 0.009, OR = 6.782,95% CI: 1.600-28.746), FIB (P = 0.001, OR = 2.662,95% CI: 1.471-4.818), and LPa (P = 0.041, OR = 1.002,95% CI: 1.000-1.004) were independent risk factors for PCAD. A total of 348 up-regulated proteins and 92 down-regulated proteins were screened by bioinformatics analysis. The occurrence of PCAD is associated with protein synthesis, intercellular communication, molecular interactions, ribosomal metabolism, glyoxylate and dicarboxylic acid metabolic pathways. Ribosomal and translational proteins influence the development of PCAD. CONCLUSION: In this study, we found that gender, FIB, and LPa are risk factors for PCAD. The analysis identified 348 up-regulated and 92 down-regulated proteins. Among them, the differentially expressed proteins DHX9, F7, APCS, and PROC were closely related to the biological process of PCAD. The screened ribosomal and translational proteins showed high-frequency associations in protein-protein interaction networks, providing potential differentially expressed proteins for a deeper understanding of the disease.
Cai et al. (Wed,) conducted a case-control in Premature coronary artery disease (n=129). Fibrinogen (FIB) was evaluated on Premature coronary artery disease (PCAD) (OR 2.662, 95% CI 1.471-4.818, p=0.001). Elevated fibrinogen (OR 2.662), male gender, and elevated lipoprotein(a) were identified as independent risk factors for premature coronary artery disease, alongside 440 differentially expressed proteins.