The decontamination efficacy of pulsed electric field in food: Establishing global kinetic parameters

Pulsed electric field (PEF) is a promising technology for microbial decontamination of liquid products such as fruit juices and beverages. This study developed a database with 569 PEF experiments for Escherichia coli, Listeria spp., Pseudomonas spp., Staphylococcus aureus, Saccharomyces cerevisiae, Salmonella spp., and lactic acid bacteria (LAB) in a range of food matrices. The efficacy of PEF for different microbial species was evaluated through the decimal-reduction volumetric energy input DEvol, which is the energy input required to achieve a one log10 microbial reduction. The matrix composition had a significant impact on the DEvol, decreasing the DEvol in high-acid fruit juices and increasing it in high-acid alcoholic products, revealing effects beyond acidity alone. Data for most species were available for high-acid fruit juices, and in this matrix, S. cerevisiae was the most sensitive species. A decision tree with eight matrix categories was developed to estimate the volumetric energy input needed for a 5-log10 reduction for different target microbial species in different matrices. The global kinetic parameters reported in this study provide a quantitative benchmark for food business operators and risk assessors, and can serve as a first indication of PEF treatment efficacy.